2009-06-11-世界卫生组织-WHO_HPV_LabNet_-_Newsletter_04_9页_260kb

WHO HPV LabNet Newsletter No.4 (June 12, 2009) serves as a comprehensive update on the network's activities. Key points include:

1. HPV LabNet Activities Overview

• Activities cover global health priorities set by the World Health Organization (WHO), with the latest development being the fourth update of the six-monthly newsletter. It highlights standardization efforts of HPV diagnostic procedures globally.

2. Availability of International Standards and Reference Reagents

• Information is available on WHO International Standards (IS) for HPV 16 DNA (06/202), HPV 18 DNA (06/206), and anti-HPV 16 antibodies (05/134). Orders can be placed via the NIBSC website, with national control laboratories exempt from charges.

3. Typical Activities of a Regional Reference Laboratory (RRL) in Thailand

• The RRL influences policy by contributing epidemiological data and providing quality assurance, HPV surveillance, and collaborations to improve cervical cancer prevention.

4. Non-commercial PCR Reverse Blotting HPV Genotyping Assay

• Based on the suggestion from a previous meeting, WHO prioritizes affordable in-house HPV typing assays to address the high costs of commercial kits. The PGMY assay from the CHUV RRL is a candidate for widespread technical transfer.

5. Establishment of an Indian HPV Vaccination Registry

• India's regulatory authority is developing national registries for HPV vaccines, aiming to standardize processes, referencing Australia’s model.

6. Second WHO HPV DNA Proficiency Study (2009)

• This study assessed the performance of 54 institutions on detecting fourteen high-risk and low-risk HPV types. Linear Array performed best among commercial tests; in-house assays showed varying sensitivity.

7. Recent Meeting Updates

• The 5th International Conference of Papillomaviruses provided a platform for discussing research advances and HPV control strategies, attracting over 2000 participants.

8. Useful Web Links

• Links are provided for WHO resources, HPV reference networks, and related international organizations (IARC, WHO Centers).

9. Contributions for Next Newsletter

• WHO invites submissions from global partners, focusing on local initiatives, technical projects, and data on HPV DNA and serosurveillance, recurring every six months.

10. List of Participating HPV LabNet Laboratories (May 2009)

• Appended list includes Global Reference Laboratories (GRL) based in Sweden, USA, and UK, and regional RRLs and associated institutions.

11. Activities of WHO HPV LabNet Global Reference Laboratories

• GRL (Sweden): Developed standardized protocols for HPV DNA testing and coordinated proficiency panels. Usage of SiHa and HeLa cell lines and VLP preparations is emphasized.
• GRL (USA CDC): Preparing a standardized HPV Laboratory Manual. Neutralizing antibody assays were analyzed, aiming to define quality control standards.

The network emphasizes capacity building, standardization of assays, and ensuring proper use of international standards to support HPV control programs globally.