2008-12-15-世界卫生组织-WHO_HPV_LabNet_-_Newsletter_03_9页_577kb

WHO HPV LabNet Newsletter Issue 3 (December 2008) Summary

This newsletter provides a brief update on the World Health Organization's (WHO) Human Papillomavirus (HPV) Laboratory Network (LabNet), highlighting key activities, priorities, and future initiatives. Below is a concise summary of the main content:

• Preface: This is the third 6-monthly newsletter from the WHO HPV LabNet, intended to offer an overview of the network's activities and progress.

• 2008 Priorities Follow-Up: The main focuses were Capacity Building and Standardization of HPV DNA and serology assays. Sweden's HPV Global Reference Laboratory conducted a proficiency study and a collaborative study on HPV16 serology. Switzerland's HPV Regional Reference Laboratory is involved in technology transfer of a non-commercial genotyping assay. These efforts aimed to ensure regional labs can perform testing and improve SOPs for an HPV Laboratory Manual. The National Institute for Biological Standards and Control (NIBSC) led work on establishing International Standards (IS) for HPV DNA and antibodies, resulting in the first ISs for HPV16 and HPV18 DNA at the WHO Biological Standards Expert Committee meeting in October 2008. Other developments include DNA ISs for HPV types 31, 33, 45, 52, and 58, with a focus on harmonizing assays and reducing lot-to-lot variation.

• Call for Contributions: WHO is seeking donations of high-quality HPV16 and/or HPV18 VLPs (Virus-Like Particles) and well-characterized serum reference samples to facilitate standardized serology testing. These are needed for HPV antibody and DNA impact studies, particularly for vaccine monitoring. Donations should be by January 2009, with NIBSC handling the distribution and studies, while ensuring confidentiality and recognition of donors.

• Evaluation of HPV16 Serology: A collaborative study using direct VLP-ELISA showed consistent results among labs, with most positive sera correctly identified. The study helped refine SOPs for an HPV Laboratory Manual and suggested preliminary consensus cut-offs.

• International Standards: The first HPV DNA ISs for types 16 and 18 were adopted, with assigned potency in IU/mL and traceability options. Discussions on standardizing units (e.g., GEq/ml) were noted. NIBSC is developing large-scale calibration materials like C33A DNA diluents and HPV DNA panels for high-risk types.

• Congratulations to Prof. Harald zur Hausen: WHO LabNet congratulates Dr. zur Hausen for his 2008 Nobel Prize in Physiology or Medicine for his discovery that HPV causes cervical cancer. His research laid the foundation for vaccines and cervical cancer prevention.

• Recent Meeting Updates:

  • WHO HPV Vaccine Advisory Committee (HVAC): July 2008 meeting reviewed new vaccine data, policy developments for cervical cancer prevention, and recommendations for monitoring vaccination programs. WHO emphasized tiered monitoring approaches tailored to country capacities.
  • WHO HPV LabNet Meeting: November 2008 meeting focused on reviewing collaborative studies, revising an HPV Laboratory Manual draft, planning future studies, and addressing sustainability. Key outcomes include incorporating evaluated protocols into the manual and initiating studies on VLP-ELISA and HPV neutralization assays. WHO highlighted the need for long-term funding and international guidelines for surveillance.

• Upcoming Meetings: A conference on HPV in Malmö, Sweden, scheduled for May 2009, is mentioned.

• Useful Web Links: Provided links to WHO resources on immunization, biologicals, HPV LabNet details, IARC, and NIBSC products for further information.

• Contributions for Next Newsletter: WHO solicits local initiatives, projects, prevalence data, and genotype-specific serosurveillance for the next newsletter edition, with contributions due within four months.

Overall, the newsletter underscores WHO's commitment to standardizing HPV assays, expanding surveillance capabilities, and advancing global efforts for cervical cancer prevention through laboratory research and collaboration.